The AKTA Pure Manual provides comprehensive guidance for operating, installing, and maintaining the AKTA Pure chromatography system, ensuring safe and efficient use in laboratory settings․
Overview of the AKTA Pure Chromatography System
The AKTA Pure chromatography system is a high-performance tool designed for efficient purification of proteins, peptides, and nucleic acids․ It offers modular flexibility, catering to both research and industrial applications․ The system is available in two primary models: the AKTA Pure 25, with a flow rate of up to 25 mL/min and pressure limits of 200 bar, and the AKTA Pure 150, supporting flow rates up to 150 mL/min with a maximum pressure of 50 bar․ Both models are controlled by the Unicorn software, ensuring precise automation and user-friendly operation․ This system is ideal for laboratories requiring versatility and scalability in chromatography processes․
Purpose and Scope of the Manual
This manual serves as a detailed guide for the safe installation, operation, and maintenance of the AKTA Pure chromatography system․ It provides step-by-step instructions to ensure optimal performance and longevity of the equipment․ The document covers essential procedures, troubleshooting, and best practices for users․ It is intended for researchers, laboratory technicians, and system administrators responsible for the instrument․ By following this manual, users can maximize the system’s efficiency and ensure reliable results in various chromatography applications․ Compliance with the guidelines is crucial for user safety and system integrity․
System Specifications and Features
The AKTA Pure 25 and 150 models offer flow rates from 0․001 to 150 ml/min and pressures up to 200 bar, featuring modular design and precise control via Unicorn software for enhanced chromatography performance․
Flow Rate and Pressure Limits
The AKTA Pure system offers two primary models: the Pure 25 and Pure 150․ The Pure 25 operates at a maximum flow rate of 25 ml/min with a pressure limit of 200 bar, making it ideal for high-pressure applications․ In contrast, the Pure 150 model supports flow rates up to 150 ml/min, with a lower pressure limit of 50 bar, suited for larger-scale processes․ These specifications ensure optimal performance across various chromatography applications, balancing speed, precision, and system safety․ Proper adherence to these limits is crucial for maintaining instrument integrity and achieving reliable results․
Key Components and Modules
The AKTA Pure system features essential modules designed for precise chromatography operations․ The core components include high-precision pumps, injection valves (e․g․, V9M-J), and detectors like the UV-900 for real-time monitoring of eluent․ The system also incorporates a flexible flow path design and a Superloop for sample handling․ The modular architecture allows seamless integration of additional components, such as columns and fraction collectors, tailored to specific applications․ These modules work harmoniously under Unicorn software control, enabling advanced process monitoring and data analysis․ Regular maintenance of these components ensures optimal system performance and longevity․
Software Control with Unicorn
The AKTA Pure system is controlled by Unicorn software, which offers advanced features for method development, data acquisition, and system operation․ Unicorn enables users to create and execute custom chromatography methods, monitor real-time data, and analyze results seamlessly․ It supports automated control of pumps, valves, and detectors, ensuring precise and reproducible runs․ The software also provides tools for troubleshooting, maintenance scheduling, and generating reports․ With intuitive interfaces and comprehensive functionality, Unicorn enhances the efficiency and scalability of chromatography processes in both research and industrial settings․ Regular updates ensure compatibility and optimal performance․
Installation and Setup
The AKTA Pure system requires careful unpacking, hardware installation, and software configuration to ensure optimal performance․ Proper calibration and preparation are essential before initial operation․
Unpacking and Hardware Installation
Carefully unpack the AKTA Pure system, ensuring all components are included and free from damage․ Place the system on a stable, level surface․ Connect the pumps, detectors, and valves according to the provided diagrams․ Ensure all tubing is securely attached and properly routed․ Install any additional modules, such as the injection valve or UV detector, following the manufacturer’s guidelines․ Connect the system to a power source and ensure all cables are safely secured․ Refer to the manual for specific instructions on levelling the system and preparing it for initial use․
Software Installation and Configuration
Install the Unicorn software on a compatible computer, ensuring the latest version is downloaded from the official source․ Follow the installation wizard to complete the setup․ Once installed, launch Unicorn and navigate to the configuration menu․ Select the appropriate system parameters for your AKTA Pure model (e․g․, Pure 25 or Pure 150)․ Configure the communication settings to establish a connection between the software and hardware․ Calibrate the system instruments, such as pumps and detectors, according to the on-screen instructions․ Refer to the manual for detailed guidance on software setup and system calibration․
System Calibration and Preparation
Before operating the AKTA Pure, ensure all components are properly calibrated․ Start by priming the pumps to remove air bubbles, using the purge syringe provided․ Next, configure the flow path according to the desired method, ensuring all tubing connections are secure․ Calibrate the detectors and injectors to verify accuracy and consistency․ Use the Unicorn software to run a system check and confirm all parameters are within operational limits․ Finally, perform a blank run to ensure the system is functioning correctly and ready for sample processing․
Operating the AKTA Pure System
The AKTA Pure system is controlled via Unicorn software, enabling seamless method execution, real-time monitoring, and precise control of chromatography processes for optimal results․
Starting a Run
To initiate a run on the AKTA Pure system, ensure all components are properly connected and configured․ Open the Unicorn software and select the appropriate method, defining parameters such as flow rate and pressure․ Prime the pumps to eliminate air bubbles and set the injection valve to the ‘Load’ position to fill the sample loop․ Connect the buffers and column, verifying all tubing connections․ Start the run via the software and monitor the process closely to address any alarms or issues promptly․
Manual mode on the AKTA Pure allows users to control individual components directly, enabling precise adjustments during chromatography runs․ Access manual controls through the Unicorn software, where you can adjust flow rates, pressure, and valve positions․ For instance, the injection valve can be switched to ‘Manual Load’ to fill sample loops externally․ This mode is ideal for troubleshooting or customizing runs without predefined method constraints․ Always ensure system stability before making manual adjustments and refer to the user manual for specific instructions to avoid operational errors․
The AKTA Pure system facilitates precise sample injection through its injection valve and sample loops․ Users can select loop sizes ranging from microliters to milliliters, depending on the model․ To configure, ensure the injection valve is in the correct position and the loop is filled manually or via the pump․ Proper configuration ensures accurate sample delivery to the column, optimizing separation efficiency․ Refer to the manual for specific instructions on loading and configuring loops for different sample volumes and chromatography methods․ The AKTA Pure system allows real-time monitoring of chromatography runs through the Unicorn software․ Users can track flow rates, pressures, UV absorption, and conductivity․ Set alarms for pressure limits, flow rates, and detector signals to ensure process control․ The software provides detailed graphs and data logs for analysis․ Manual intervention is possible via the control panel or software interface․ Regularly monitor system status, alarms, and performance metrics to ensure optimal operation and troubleshoot issues promptly․ Proper monitoring ensures consistent results and system reliability during chromatography processes․ Regular maintenance and troubleshooting ensure optimal performance and extend system lifespan․ This section provides procedures for resolving common issues and performing routine upkeep effectively․ Regular maintenance ensures optimal performance and longevity of the AKTA Pure system․ Replace tubing kits and check pump seals periodically․ Clean components and verify connections․ Follow the manufacturer’s guidelines for replacing worn parts․ Document all maintenance activities for traceability․ Neglecting routine upkeep may lead to system downtime or decreased accuracy․ Always refer to the official manual for detailed procedures tailored to your specific model, such as Pure 25 or Pure 150․ Proper scheduling prevents unexpected issues and ensures reliable operation․ Common issues with the AKTA Pure include pressure alarms, tubing leaks, and injection valve malfunctions․ Pressure alarms often indicate obstructions or excessive backpressure․ Inspect and clean connections, and ensure columns are properly packed․ Tubing leaks can be resolved by replacing worn or damaged tubing․ For injection valve issues, check the sample loop configuration and ensure manual mode is correctly engaged․ Refer to the manual for specific troubleshooting steps and solutions to minimize downtime and maintain system efficiency․ Regular maintenance can prevent many of these issues from arising․ Regular replacement of tubing and consumables ensures optimal performance of the AKTA Pure system․ Replace tubing if signs of wear, cracks, or leaks are observed․ Use the recommended tubing kit for your specific model․ To replace, depressurize the system, disconnect the old tubing, and install the new one securely․ For consumables like sample loops or injection valves, refer to the manual for compatible parts․ Ensure all connections are tight to prevent leaks․ Proper replacement procedures maintain system integrity and prevent operational issues․ Always follow the manufacturer’s guidelines for compatibility and installation․ Always wear protective equipment when handling high-pressure components․ Avoid exposure to hazardous materials and follow proper shutdown procedures․ Adhere to manufacturer guidelines for safe operations․ Always wear protective equipment, including gloves and safety goggles, when handling high-pressure components or chemicals․ Ensure the system is properly grounded to prevent electrical hazards․ Follow the manufacturer’s guidelines for operating pressures and flow rates to avoid system damage or personal injury․ Keep the work area clean and well-ventilated, especially when handling potentially hazardous materials․ Regularly inspect tubing and connections for wear or leaks․ Adhere to all safety warnings and precautions outlined in the manual to ensure safe and reliable operation of the AKTA Pure system․ When handling high-pressure components, ensure all connections are secure to prevent leaks or component damage․ Use approved tools to tighten fittings to the recommended torque specifications․ Avoid over-tightening, which may damage threads․ Always depressurize the system before servicing or replacing high-pressure parts․ Wear protective eyewear and gloves to safeguard against potential fluid discharge․ Follow the manual’s specific instructions for high-pressure component maintenance to ensure safety and system integrity․ Regular inspections are crucial to identify and address wear or damage promptly․ In case of an emergency, immediately depressurize the system and disconnect power․ Follow the shutdown procedure outlined in the manual to ensure safe cessation of operations․ If a high-pressure component fails, evacuate the area and wear protective gear․ Contact trained personnel or support for assistance․ Never attempt repairs without proper training․ Keep emergency contact information readily available․ Always prioritize personal safety and avoid hazardous situations․ Regular training on emergency procedures is essential for all users of the AKTA Pure system․ Ensure all safety protocols are strictly followed․ This section provides access to user manuals, technical specifications, and online support for the AKTA Pure system, ensuring comprehensive guidance and troubleshooting assistance․ The ÄKTA Pure User Manual is a primary resource for understanding system operation, installation, and maintenance․ It provides detailed instructions for configuring modules, troubleshooting common issues, and replacing components like tubing kits and injection valves․ The manual also covers software integration with Unicorn, enabling users to control and monitor chromatography processes effectively․ Additional guides include technical specifications, troubleshooting sections, and best practices for system calibration․ Regularly updated, these documents ensure users can optimize the performance and longevity of their ÄKTA Pure system․ The technical specifications and data sheets for the ÄKTA Pure system provide detailed information on its operational capabilities, including flow rates of up to 25 ml/min (Pure 25) and 150 ml/min (Pure 150), with maximum pressures of 200 bar and 50 bar, respectively․ These documents outline key components such as pumps, injection valves, and compatible software․ Data sheets also cover system dimensions, weight, and compatibility with various chromatography accessories․ They serve as essential references for ensuring optimal performance, maintenance, and compliance with operational guidelines․ The ÄKTA Pure system is supported by extensive online resources, including user manuals, troubleshooting guides, and interactive forums․ These platforms provide access to detailed documentation, software updates, and technical support․ Users can engage with a global community of chromatography experts and peers to share knowledge and resolve challenges․ Additionally, official support channels offer rapid assistance for operational and maintenance inquiries․ Online forums also feature discussions on best practices, optimizing system performance, and addressing common issues, ensuring users maximize their experience with the ÄKTA Pure chromatography system․Manual Mode Operations
Sample Injection and Loop Configuration
Monitoring and Controlling the Process
Maintenance and Troubleshooting
Scheduled Maintenance Procedures
Common Issues and Solutions
Replacing Tubing and Consumables
Safety Precautions and Guidelines
General Safety Instructions
Handling High-Pressure Components
Emergency Procedures
Documentation and Resources
User Manual and Guides
Technical Specifications and Data Sheets
Online Support and Forums